Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm–egg fusion in mice
نویسندگان
چکیده
منابع مشابه
Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm–egg fusion in mice
Sperm-egg fusion is indispensable for completing mammalian fertilization. Although the underlying molecular mechanisms are poorly understood, requirement of two spermatozoon factors, IZUMO1 and SPACA6, and two oocyte factors, CD9 and the IZUMO1 counter-receptor JUNO, has been proven by gene disruption, and the binding of cells to an oocyte can be reconstituted by ectopic expression of IZUMO1. H...
متن کاملMolecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion.
Although the membrane fusion of spermatozoon and egg cells is the central event of fertilization, the underlying molecular mechanism remains virtually unknown. Gene disruption studies have showed that IZUMO1 on spermatozoon and CD9 on oocyte are essential transmembrane proteins in sperm-egg fusion. In this study, we dissected IZUMO1 protein to determine the domains that were required for the fu...
متن کاملThe mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion.
An average human ejaculate contains over 100 million sperm, but only a few succeed in accomplishing the journey to an egg by migration through the female reproductive tract. Among these few sperm, only one participates in fertilization. There might be an ingenious molecular mechanism to ensure that the very best sperm fertilize an egg. However, recent gene disruption experiments in mice have re...
متن کاملMale infertility-related molecules involved in sperm-oocyte fusion
Male infertility has become a very serious problem in the human reproduction system, but the molecular mechanism of infertility remains largely unknown. Fertilization is the phenomenon in which a sperm and oocyte find each other, interact, and fuse. Sperm-oocyte fusion-related factors on the sperm side play crucial roles in male infertility. For example, IZUMO1 is well-known as a sperm protein ...
متن کاملVisualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.
Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent pr...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2015
ISSN: 2041-1723
DOI: 10.1038/ncomms9858